منابع مشابه
The budding yeast endocytic pathway.
Recent live-cell imaging studies, coupled with powerful genetic, biochemical and pharmacological tests of function, have expanded our understanding of the molecular events that underlie clathrin/actin mediated-endocytosis in budding yeast. Many features of this pathway are evolutionarily conserved (Engqvist-Goldstein and Drubin, 2003; Kaksonen et al, 2006). Therefore, insights into the intricat...
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In order for haploid gametes to fuse during fertilization, microtubules (MTs) must generate forces that are sufficient to move the nuclei together. Nuclear movements during fertilization rely on microtubule-associated proteins (MAPs), many of which have been characterized extensively during mitosis. A useful model system to study MT-dependent forces before nuclear fusion, or karyogamy, is the m...
متن کاملEndocytic internalization in budding yeast requires coordinated actin nucleation and myosin motor activity.
Actin polymerization essential for endocytic internalization in budding yeast is controlled by four nucleation promoting factors (NPFs) that each exhibits a unique dynamic behavior at endocytic sites. How each NPF functions and is regulated to restrict actin assembly to late stages of endocytic internalization is not known. Quantitative analysis of NPF biochemical activities, and genetic analys...
متن کاملRNAi in budding yeast.
RNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae. Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small interfering ...
متن کاملImaging Polarization in Budding Yeast.
We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected ...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 2006
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.03251